Bench Top AFLA

Aquatic Laser Fluorescence Analyzer (ALFA)

WET Labs' Aquatic Laser Fluorescence Analyzer (ALFA) provides spectrally and temporally resolved measurements of key bio-environmental variables in oceanic, coastal, and fresh waters.
WET Labs’ Aquatic Laser Fluorescence Analyzer (ALFA) provides spectrally and
temporally resolved measurements of key bio-environmental variables in oceanic,
coastal, and fresh waters. Operation modes allow underway shipboard measurements
and discrete sample analysis for accurate assessments of pigment biomass,
phytoplankton community structure and physiology.
• Spectral deconvolution of overlapped fluorescence bands with Raman normalization
• Improved assessment of chlorophyll concentrations for estimating phytoplankton biomass
• Discrimination of 5 phycobiliprotein pigments for structural phytoplankton characterization
• Fluorescence assessment of chromophoric dissolved organic matter (CDOM) 
• Measures variable fluorescence, Fv/Fm, with correction for background CDOM fluorescence
• Instrument software allows real-time data analysis and display with GPS coordinates and time



Excitation Light Source405 nm and 510 nm TEC lasers
Spectral Emission Detection400 – 800 nm, CCD array spectrometer
Variable Fluorescence DetectionAuto ranging PMT and 12 bit waveform digitizer, 0.01 mg m-3 Chl a
Pulse ControlProgrammable/automatic control modes, 50 μs – 1000 μs, 0.1-30 Hz
Sampling RateVariable integration time, 0.1 s – 10 s (5 s typical)
Operating PlatformWindows
Power Requirements100-240 V AC, 24 W
Operating Temperature4 deg C to +40  deg C
Dimensions (L x W X H)36 cm x 41 cm x 15 cm (14” x 16” X 6”)
Weight (in air)5.4 kg (12 lbs)
Configuration ModesContinuous flow-through measurements or discrete sample analysis
Water Sample Connectors1/4” quick connect fittings
Measurement Sample Volume~1cm3
Flow RateUp to 1L/min (continuous), or ~100mL/min (pumped)
PumpIntegrated peristaltic
Housing MaterialsPowder coated aluminum case
Computer InterfaceUSB connector


Product Brochure

ALFA Instrument Introduction and Applications

Tuesday, January 28, 2014
Product Brochure

ALFA Startup Guide

Monday, June 24, 2013
Product Brochure

ALFA Specification Sheet

Friday, February 10, 2012
Product Datasheet

ALFA datasheet

Monday, December 5, 2016
White Paper


Tuesday, January 1, 2013
White Paper

Next Generation of Advanced Laser Fluorescence Technology for Characterization of Natural Aquatic Environments

Sunday, December 1, 2013
White Paper

Analysis of spectral excitation for measurements of fluorescence constituents in natural waters

Monday, December 2, 2013
White Paper

Underway sampling of marine inherent optical properties on the Tara Oceans expedition as a novel resource for ocean color satellite data product validation

Wednesday, December 11, 2013
Werdell-et-al-2014_LO Methods-Inpress.pdf
White Paper

The characteristics of particulate absorption, scattering and attenuation coefficients in the surface ocean; Contribution of the Tara Oceans expedition

Saturday, December 14, 2013
Boss-et-al-2014_LO Methods-Inpress.pdf
White Paper

Optical proxy for phytoplankton biomass in the absence of photophysiology: Rethinking the absorption line height

Thursday, January 23, 2014
Roesler-Barnard-2014_LO Methods-Inpress.pdf
White Paper

Aquatic Laser Fluorescence Analyzer: Field Evaluation in the Northern Gulf of Mexico

Friday, August 29, 2014
Scientific Poster

ALFA A New Instrument for Characterization of Natural Aquatic Environments

Thursday, February 21, 2013
Barnard ALFA - Ocean-Sciences-2012-Research-Poster.pdf
Scientific Poster

Advanced Laser Fluorometry (ALF): New Technology for Oceanography and Environmental Monitoring

Friday, February 21, 2014
Technical Note

ALFA Bottle Cap Tech Note

Monday, June 24, 2013

What should I use to lubricate bulkhead connectors?

WET Labs recommends keeping the connectors on your meter lubricated using a good quality silicone spray lubricant, such as 3M Silicone Spray or Loctite 8021 spray. These can be purchased at your local hardware store. Avoid silicone grease. It is messy and attracts contaminants. 

Do not use WD-40. It will destroy connectors.